epitope mapping within an internal region of ACOT12 of human origin
recommended for detection of ACOT12 of mouse, rat and human origin by WB, IF and ELISA; non cross-reactive with other ACOT family members; also reactive with additional species, including equine and canine
ACOT12 Background Information Acyl-CoA thioesterases (ACOTs) are a group of enzymes that catalyze the hydrolysis of acyl-CoA to form coenzyme A (CoA) and a free fatty acid. Through their catalytic activity, ACOTs are able to regulate the level of fatty acids and acyl-CoAs within the cell. ACOT12 (acyl-CoA thioesterase 12), also known as CACH, CACH1 or STARD12, is a 555 amino acid protein that localizes to the cytoplasm and contains one START domain and two acyl coenzyme A hydrolase domains. Existing as either a homodimer or a homotetramer, ACOT12 plays a role in pyruvate metabolism, specifically by catalyzing the hydrolysis of acetyl-CoA to acetate and CoA. The gene encoding ACOT12 maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome.
