epitope mapping near the C-terminus of NELF-E of human origin
recommended for detection of NELF-E of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine and porcine
NELF-E Background Information NELF-E, for negative elongation factor E, is a putative RNA binding protein (1). NELF-E is one of the five components of the multisubunit NELF complex that cooperates with DSIF to repress RNA polymerase II elongation (1). Control of transcription elongation requires a complex interplay between positive transcription elongation factor b (P-TEFb) and negative transcription elongation factors, DSIF and NELF (2). DSIF and NELF, act as negative transcription elongation factors by increasing the time the polymerase spends at pause sites (3). DSIF/NELF inhibition of transcription is prevented by P-TEFb in cooperation with FACT (4). NELF-E is also known as RD and RDBP (RD RNA-binding protein). (5,6). RD, the acronym of the most common dipeptide repeat describes the single letter symbols for arginine (R) and aspartic acid (D), respectively (5). NELF-E has a functional RNA-binding domain, whose mutations impair transcription repression without affecting known protein-protein interactions (7). The human NELF-E gene maps to chromosome 6p21.3 and encodes a 371 amino acid protein (5).
