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Neu siRNA (h), shRNA and Lentiviral Particle Gene Silencers

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See Neu Antibodies for analysis of cellular responses to Neu gene silencers, including Neu siRNA, Neu shRNA Plasmids and Neu shRNA Lentiviral Particles.

Also see Neu Inhibitors for functional analysis of cellular responses to Neu.


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 注文情報
製品名カタログ #Unit価格数量追加Favorites
Neu siRNA (h) sc-29405 10 µM $258
Neu (h)-PR sc-29405-PR 10 µM, 20 µl $23
Neu shRNA Plasmid (h) sc-29405-SH 20 µg $520
Neu shRNA (h) Lentiviral Particles sc-29405-V 200 µl $625

Neu siRNA (h): sc-29405

 |  siRNA データシート / プロトコール
  • 10 µM, 50-100 transfections
  • siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
  • for independent verification of Neu gene silencing results, individual siRNA duplex components are also available upon request
  • suitable control antibody: Neu (C-18): sc-284
  • sqRT-PCR Primer also available: Neu (h)-PR: sc-29405-PR, 20 µl
  • See 3 citations for Neu siRNA (h)
 
 お奨めsiRNAサポート製品 (click product name for more information)
製品名カタログ #Unit価格数量追加Favorites
siRNA Dilution Buffer sc-29527 1.5 ml $5
siRNA Transfection Reagent sc-29528 0.3 ml $142
siRNA Transfection Medium sc-36868 20 ml $9
siRNA Reagent System sc-45064 1 kit $331
Control siRNA-A sc-37007 10 µM $77
Control siRNA (FITC Conjugate)-A sc-36869 10 µM $104
Neu shRNA Plasmid (h): sc-29405-SH shRNAデータシート / プロトコール
  • 20 µg, up to 20 transfections
  • shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
  • shRNA sequences correspond to Neu siRNA Gene Silencer sequences
  • provided as transfection-ready purified plasmid DNA target-specific gene silencing
  • after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
  • shRNA Plasmids are intended only for non-commercial use. Click here for details.
  • suitable control antibody: Neu (C-18): sc-284
  • sqRT-PCR Primer also available: Neu (h)-PR: sc-29405-PR, 20 µl
 お奨めshRNAサポート製品 (click product name for more information)
製品名カタログ #Unit価格数量追加Favorites
shRNA Plasmid Transfection Reagent sc-108061 0.2 ml $71
shRNA Plasmid Transfection Medium sc-108062 20 ml $8
Control shRNA Plasmid-A sc-108060 20 µg $83
Control shRNA Plasmid-B sc-108065 20 µg $83
Control shRNA Plasmid-C sc-108066 20 µg $83
copGFP Control Plasmid sc-108083 20 µg $83
Puromycin dihydrochloride sc-108071 25 mg $30
 
Neu shRNA (h)
Lentiviral Particles: sc-29405-V
Lentiviral Particles Datasheet / プロトコール
  • 200 µl viral stock containing 1x 106 infectious units of virus (IFU) , sufficient for 10-20 transductions
  • Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
  • Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
  • shRNA sequences correspond to Neu siRNA siRNA Gene Silencer sequences
  • After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
  • Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
  • Lentiviral Particles are intended for non-commercial use. Click here for details.
 お奨めレンチウイルスパーティクルのサポート製品f (click product name for more information)
製品名カタログ #Unit価格数量追加Favorites
Control shRNA Lentiviral Particles-A sc-108080 200 µl $176
copGFP コントロールレンチウイルス粒子 sc-108084 200 µl $176
Puromycin dihydrochloride sc-108071 25 mg $30
Polybrene® sc-134220 10 mg/ml $18
 お奨めのコントロール抗体 (click product name for more information)
製品名カタログ #Unit価格数量追加Favorites
Neu Antibody (C-18) sc-284 200 µg/ml $279
goat anti-rabbit IgG-HRP sc-2004 200 µg/0.5 ml $70
 
お奨めサポート品
(お好みのアプリケーションボタンをクリックして下さい)
 
Species 遺伝子名 Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
ヒト ERBB2 2064 17q12 NM_001005862, NM_004448 P04626
211980
 
Neu siRNA (h) 参考文献
他の研究者がNeu siRNA (h)をどのように使用したかを見る。


3 total citations
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Neu siRNA (h) Data
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Neu siRNA (h): sc-29405. Western blot analysis of Neu expression in non-transfected control (A) and Neu siRNA transfected (B) A-431 cells. Blot probed with Neu (C-18): sc-284. α-actinin (H-2): sc-17829 used as specificity and loading control.
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データシート: siRNA, shRNA, Lentiviral Particles
プロトコール: siRNA, shRNA, Lentiviral Particles