Neu siRNA (h), shRNA and Lentiviral Particle Gene Silencers

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See Neu Antibodies for analysis of cellular responses to Neu gene silencers, including Neu siRNA, Neu shRNA Plasmids and Neu shRNA Lentiviral Particles.

Also see Neu Inhibitors for functional analysis of cellular responses to Neu.

注文情報

製品名	カタログ #	Unit	価格
Neu siRNA (h)	sc-29405	10 µM	$258
Neu (h)-PR	sc-29405-PR	10 µM, 20 µl	$23
Neu shRNA Plasmid (h)	sc-29405-SH	20 µg	$520
Neu shRNA (h) Lentiviral Particles	sc-29405-V	200 µl	$625

Neu siRNA (h): sc-29405	\| siRNAデータシート / プロトコール

10 µM, 50-100 transfections
siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression
for independent verification of Neu gene silencing results, individual siRNA duplex components are also available upon request
suitable control antibody: Neu (C-18): sc-284
sqRT-PCR Primer also available: Neu (h)-PR: sc-29405-PR, 20 µl
See 3 citations for Neu siRNA (h)

お奨めsiRNAサポート製品 (click product name for more information)

製品名	カタログ #	Unit	価格
siRNA Dilution Buffer	sc-29527	1.5 ml	$5
siRNA Transfection Reagent	sc-29528	0.3 ml	$142
siRNA Transfection Medium	sc-36868	20 ml	$9
siRNA Reagent System	sc-45064	1 kit	$331
Control siRNA-A	sc-37007	10 µM	$77
Control siRNA (FITC Conjugate)-A	sc-36869	10 µM	$104

Neu shRNA Plasmid (h): sc-29405-SH	shRNAデータシート / プロトコール

20 µg, up to 20 transfections
shRNA Plasmids generally consist of a pool of three to five lentiviral vector plasmids each encoding target-specific 19-25 nt (plus hairpin) shRNAs designed to knockdown gene expression
shRNA sequences correspond to Neu siRNA Gene Silencer sequences
provided as transfection-ready purified plasmid DNA target-specific gene silencing
after transfection, cells stably expressing shRNAs can be isolated by puromycin selection
shRNA Plasmids are intended only for non-commercial use. Click here for details.
suitable control antibody: Neu (C-18): sc-284
sqRT-PCR Primer also available: Neu (h)-PR: sc-29405-PR, 20 µl

Neu shRNA (h) Lentiviral Particles: sc-29405-V	Lentiviral Particles Datasheet / プロトコール

200 µl viral stock containing 1x 10⁶ infectious units of virus (IFU) , sufficient for 10-20 transductions
Lentiviral Particles are provided as transduction-ready viral particles for gene silencing
Lentiviral Particles generally contain three to five expression constructs each encoding target-specific 19-25 nt (plus hairpin) shRNA designed to knockdown gene expression
shRNA sequences correspond to Neu siRNA siRNA Gene Silencer sequences
After transduction, stable cell lines expressing the shRNA may be isolated via selection with puromycin
Biosafety - Lentiviral Particles are replication-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. Click here for details.
Lentiviral Particles are intended for non-commercial use. Click here for details.

お奨めレンチウイルスパーティクルのサポート製品f (click product name for more information)

製品名	カタログ #	Unit	価格
Control shRNA Lentiviral Particles-A	sc-108080	200 µl	$176
copGFP コントロールレンチウイルス粒子	sc-108084	200 µl	$176
Puromycin dihydrochloride	sc-108071	25 mg	$30
Polybrene^®	sc-134220	10 mg/ml	$18

お奨めのコントロール抗体 (click product name for more information)

製品名	カタログ #	Unit	価格	数量	追加
Neu Antibody (C-18)	sc-284	200 µg/ml	$279
goat anti-rabbit IgG-HRP	sc-2004	200 µg/0.5 ml	$70

お奨めサポート品

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siRNA shRNAプラスミド shRNAレンチウィルス

Species	遺伝子名	Gene ID	Chromosome Location	Isoform (mRNA) Accession #	Protein Accession #	OMIM™ Number

ヒト	ERBB2	2064	17q12	NM_001005862, NM_004448	P04626	211980

お奨めshRNAサポート製品 (click product name for more information)

製品名	カタログ #	Unit	価格
shRNA Plasmid Transfection Reagent	sc-108061	0.2 ml	$71
shRNA Plasmid Transfection Medium	sc-108062	20 ml	$8
Control shRNA Plasmid-A	sc-108060	20 µg	$83
Control shRNA Plasmid-B	sc-108065	20 µg	$83
Control shRNA Plasmid-C	sc-108066	20 µg	$83
copGFP Control Plasmid	sc-108083	20 µg	$83
Puromycin dihydrochloride	sc-108071	25 mg	$30

Neu siRNA (h) 参考文献

他の研究者がNeu siRNA (h)をどのように使用したかを見る。

3 total citations

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Neu siRNA (h) Data

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Neu siRNA (h): sc-29405. Western blot analysis of Neu expression in non-transfected control (A) and Neu siRNA transfected (B) A-431 cells. Blot probed with Neu (C-18): sc-284. α-actinin (H-2): sc-17829 used as specificity and loading control.

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データシート: siRNA, shRNA, Lentiviral Particles

プロトコール: siRNA, shRNA, Lentiviral Particles

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