epitope mapping within an internal region of Whirlin of human origin
recommended for detection of all isoforms of Whirlin of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine and porcine
Whirlin Background Information Whirlin is a cytoplasmic PDZ domain-containing protein that plays a role in elongation and maintenance of stereocilia, mechanosensory organelles located in hair cells of the inner ear. Whirlin co-localizes with actin filaments and is primarily detected in cochlear hair cells. It is connected to the dynamic Usher protein interactome and has a pleiotropic function in both the retina and the inner ear. Myosin XVa is a motor protein that accociates with the second and third PDZ domain of Whirlin through its C-terminal PDZ-ligand. Myosin XVa then delivers Whirlin to the tips of stereocilia, which are subsequently elongated. p55 also interacts with Whirlin, and mutations in DFNB31, the Whirlin gene, lead to an early ablation of p55 labeling of stereocilia, which may cause recessive hearing loss in rats and humans.
Whirlin (D-20)
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Whirlin (D-20): sc-49785. Western blot analysis of Whirlin expression in non-transfected: sc-117752 (A) and human Whirlin transfected: sc-112679 (B) 293T whole cell lysates.
Whirlin (D-20): sc-49785. Western blot analysis of Whirlin expression in non-transfected: sc-117752 (A) and human Whirlin transfected: sc-112818 (B) 293T whole cell lysates.
