mature leukemia inhibitory factor (LIF) of mouse origin, lyophilized
available as 106 units (10 µg) (sc-4989) or 5x106 units (50 µg) (sc-4989A)
potent inhibitor of spontaneous embryonic mouse stem cell differentiation and useful for in vitro maintenance of the pluripotential phenotype of ES cells
1000 U per 1 ml of tissue culture media is required to maintain mouse ES cells with a stem cell phenotype; 106 units is sufficient for 1 L of tissue culture media, 107 units is sufficient for 10 L of tissue culture media
produced in E. coli as a GST-tagged fusion protein; GST tag is cleaved and LIF is purified by HPLC chromatography
>95% pure by SDS-PAGE and 0.22 micron sterile filtered prior to lyophilization; tested negative for mycoplasmas
Mature, biologically active, recombinant LIF is indistinguishable from native LIF in its biological activities in vitro.
Leukemia Inhibitory Factor (LIF) is an important cell culture supplement. LIF suppresses spontaneous differentiation thereby promoting long-term maintenance of embryonic stem cells. Additional LIF functions include stem cell pluripotency control, cholinergic neuron differentiation, metabolism of bone and fat, promotion of megakaryocyte production and mitogenesis of certain factor dependent cell lines in vivo.
LIF (mBA)
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Mouse LIF (sc-4989) induction of Stat3 p91 phosphorylation of Tyr 705 in untreated (A) and LIF-treated (B) 3T3-L1 cells. Antibody tested: p-Stat3 (B-7): sc-8059.
LIF (mBA): sc-4989. Undifferentiated mouse M1 cells cultured for 1 week in 0.3% agar medium without LIF (A) and differentiated mouse M1 cells cultured for 1 week in 0.3% agar medium with 0.05ng/ml of mouse LIF (B).
