DAZAP1 Background Information DAZAP1 (deleted in azoospermia-associated protein 1) is a 407 amino acid RNA-binding protein that interacts with DAZ (deleted in azoospermia), a gene with multiple protein products that are deleted in infertile men. Localized to the nucleus of round spermatids and to the cytoplasm of elongated spermatids, DAZAP1 contains two RNP motifs and is thought to be essential for normal spermatogenesis. Binding of DAZAP1 to DAZ mRNA induces translation of DAZ proteins that are required for germ cell development. When DAZAP1 is phosphorylated, it dissociates from DAZ mRNA and prevents proper protein translation, thereby regulating the expression of DAZ proteins. Additionally, DAZAP1 can fuse to the DNA-binding protein MEF-2D; a fusion that disrupts proper signaling pathways and may, therefore, be involved in leukemogenesis. DAZAP1 is expressed predominately in the testis, with weak expression observed in the thymus, heart, liver, brain and pancreas. Two isoforms of DAZAP1 exist due to alternative splicing events.
DAZAP1 (T-14)
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DAZAP1 (T-14): sc-55088. Western blot analysis of DAZAP1 expression in IMR-32 nuclear extract (A) and rat testis (B) and mouse testis (C) tissue extracts.
