Dynactin p62 Background Information Dynactin is a multisubunit complex and a required cofactor for most, or all, of the cellular processes powered by the microtubule-based motor cytoplasmic dynein (1). Dynactin contains a short actin-related protein 1 (Arp1) filament with capZ at the barbed end and p62 at the pointed end (2-5). The p62 subunit is an integral component of 20 S dynactin with a highly conserved cysteine-rich motif that interacts directly with Arp1 (2). Dynactin p62 has a punctate cytoplasmic distribution as well as centrosomal distribution typical of dynactin (1). In addition, Dynactin p62 is distributed in the nucleus at very high expression levels (1). Due to the structural composition of dynactin, the p62 subunit is implicated in Arp1 pointed-end binding and in linking dynein and dynactin to the cortical cytoskeleton (2-4).
Dynactin p62 (H-4)
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Dynactin p62 (H-4): sc-55603. Western blot analysis of Dynactin p62 expression in H4 (A), EOC 20 (B), BC3H1 (C) and Sol8 (D) whole cell lysates.
Dynactin p62 (H-4): sc-55603. Immunoperoxidase staining of formalin fixed, paraffin-embedded human kidney tissue showing cytoplasmic staining of cells in glomeruli and tubules (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Dynactin p62 (H-4): sc-55603. Western blot analysis of Dynactin p62 expression in non-transfected 293T: sc-117752 (A), mouse Dynactin p62 transfected 293T: sc-119870 (B) and BC3H1 (C) whole cell lysates.
Dynactin p62 (H-4): sc-55603. Western blot analysis of Dynactin p62 expression in RAW 264.7 whole cell lysate.
