epitope mapping within an internal region of NCB5OR of human origin
recommended for detection of NCB5OR of mouse, rat and human origin by WB, IF and ELISA; also reactive with additional species, including equine, canine, bovine and avian
NCB5OR Background Information NCB5OR, also referred to as CYB5R4 (cytochrome b5 reductase 4), is a flavohemoprotein that contains cytochrome b5 and chrome b5 reductase cytodomains. A member of the flavoprotein pyridine nucleotide cytochrome reductase family, NCB5OR is widely expressed and colocalizes with calreticulin to the endoplasmic reticulum (ER). NCB5OR has a cytochrome b5 heme-binding domain as well as one CS domain, two FAD and two iron binding motifs. NCB5OR reduces cytochrome c, methemoglobin, ferricyanide and molecular oxygen in vitro. NCB5OR is involved in the ER stress response pathway and plays a critical role in protecting pancreatic beta-cells against oxidative stress by preventing excess buildup of reactive oxygen species (ROS). The absence of NCB5OR may cause insulin-deficient diabetes.
