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ホーム » 転写制御因子 » Histone 抗体 / Histone 抗体 » Ac-Histone H3 (Lys9/14): sc-8655

Ac-Histone H3 (Lys9/14) 抗体: sc-8655

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  • goat polyclonal IgG, 200 µg/ml; also available as rabbit IgG, 200 µg/ml, sc-8655-R
  • epitope mapping near the N-terminus of Ac-Histone H3 of human origin
  • recommended for dectection of Histone H3 of mouse, rat, human, Drosphila, Xenopus and C. elegans origin acetylated at Lys 9 and Lys 14 by WB, IP, IF and ELISA; also reactive with additional species, including equine, canine, bovine, porcine and avian
  • blocking peptide, sc-8655 P
  • Suitable for use as control antibody for Histone H3 (Ser 28): sc-24516.
 
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注文情報参考文献
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Species 遺伝子名 Gene ID Chromosome Location Isoform (mRNA) Accession # Protein Accession # OMIM™ Number
ヒト H3F3A 3020 1q42.12 NM_002107 P84243
 601128
ヒト H3F3B 3021 17q25.1 NM_005324 P84243
 601058
ヒト HIST3H3 8290 1q42.13 NM_003493 Q16695
 602820
ヒト HIST1H3A 8350 6p22.1 NM_003529 P68431
 602810
ヒト HIST1H3D 8351 6p22.1 NM_003530 NP_003521
 602811
ヒト HIST1H3C 8352 6p22.1 NM_003531 P68431
 602812
ヒト HIST1H3E 8353 6p22.1 NM_003532 P68431
 602813
ヒト HIST1H3I 8354 6p22.1 NM_003533 P68431
 602814
ヒト HIST1H3G 8355 6p22.1 NM_003534 P68431
 602815
ヒト HIST1H3J 8356 6p22.1 NM_003535 P68431
 602817
ヒト HIST1H3H 8357 6p22.1 NM_003536 P68431
 602818
ヒト HIST1H3B 8358 6p22.1 NM_003537 P68431
 602819
ヒト HIST1H3F 8968 6p22.1 NM_021018 P68431
 602816
ヒト HIST2H3C 126961 1q21.2 NM_021059 Q71DI3
 142780
ヒト HIST2H3A 333932 1q21.2 NM_001005464 Q71DI3
 n/a
ヒト LOC440093 440093 12p11.21 NM_001013699 Q6NXT2
 n/a
ヒト LOC644914 644914 4q31.1 XM_930111 XP_935204
 n/a
ヒト HIST2H3D 653604 1q21.2 XM_497711 NP_001116847
マウス Hist2h3c1 15077 3 F2.1 P84228
 N/A
マウス Hist2h3c2 97114 3 F2.1 P84228
 N/A
マウス Hist1h3g 97908 13 A3.1 P68433
 N/A
マウス Hist1h3f 260423 13 A3.1 P84228
 N/A
マウス Hist1h3c 319148 13 A3.1 P84228
 N/A
マウス Hist1h3d 319149 13 A3.1 P84228
 N/A
マウス Hist1h3b 319150 13 A3.1 P84228
 N/A
マウス Hist1h3e 319151 13 A3.1 P84228
 N/A
マウス Hist1h3h 319152 13 A3.1 P68433
 N/A
マウス Hist1h3i 319153 13 A3.1 P68433
 N/A
マウス Hist2h3b 319154 3 F2.1 P84228
 N/A
マウス Hist1h3a 360198 13 A3.1 P68433
 N/A
 
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 注文情報
製品名カタログ #Unit価格数量追加Favorites
Ac-Histone H3 (Lys9/14) sc-8655 200 µg/ml $279
Ac-Histone H3 (Lys 9/14) P sc-8655 P
(peptide)
 100 µg/0.5 ml $61
Ac-Histone H3 (Lys 9/14)-R sc-8655-R 200 µg/ml $279
 WB用ポジティブコントロール細胞可溶化物 (click product name for more information)
製品名カタログ #Unit価格数量追加Favorites
NIH/3T3 Whole Cell Lysate sc-2210 500 µg/200 µl $104

Ac-Histone H3 Background Information
In eukaryotes, DNA is wrapped around histone octamers to form the basic unit of chromatin structure. The octamer is composed of histones H2A, H2B, H3 and H4, and it associates with approximately 200 base pairs of DNA to form the nucleosome. The association of DNA with histones results in dense packing of chromatin, which restricts proteins involved in gene transcription from binding to DNA. p300 preferentially acetylates Histone H3 at lysines 14 and 18 and Histone H4 at lysines 5 and 8. PCAF in its native form, primarily acetylates Histone H3 at lysine 14 to a monoacetylated form, and less efficiently acetylates Histone H4 at lysine 8. Histone H4 may also be acetylated at lysines 12 and 16, and the involvement of acetylated H4 with Histones H2A, H2B and H3 suggests that acetylated histones may be involved in dynamic chromatin remodeling.

Ac-Histone H3 (Lys9/14) 参考文献
他の研究者が、Ac-Histone H3 (Lys9/14): sc-8655抗体およびAc-Histone H3 (Lys9/14)のconjugate抗体をどのように使用したかを見る。


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Ac-Histone H3 (Lys9/14)
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sc-8655
Ac-Histone H3 (Lys 9/14): sc-8655. Western blot analysis of acetylated Histone H3 expression in NIH/3T3 whole cell lysate.
sc-8655
Trichostatin A: sc-3511. Western blot analysis of Ac-Histone H3 acetylation in untreated (A,C) and Trichostatin A treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include: Ac-Histone H3 (Lys 9/14): sc-8655 (A,B) and Histone H3 (C-16): sc-8654 (C,D).
sc-8655
Ac-Histone H3 (Lys 9/14)-R: sc-8655-R. Western blot analysis of Ac-Histone H3 acetylation in untreated (A) and Trichostatin A (sc-3511) treated (B) NIH/3T3 whole cell lysates. Note upregulation of Ac-Histone H4 expression in lane B.
sc-8655
Western blot analysis of Ac-Histone H3 acetylation in untreated (A,C) and Trichostatin A (sc-3511) treated (B,D) NIH/3T3 whole cell lysates. Antibodies tested include Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B) and Histone H3 (C-16): sc-8654 (C,D).
sc-8655
Western blot analysis of Histone H3 acetylation in untreated (A) and Trapoxin A (sc-253730) treated (B) HeLa whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Ac-Histone H3 in lane B.
sc-8655
Western blot analysis of Histone H3 acetylation in untreated (A) and M 344 (sc-203124) treated (B) NIH/3T3 whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Histone H3 in lane B.
sc-8655
Western blot analysis of Histone H3 acetylation in untreated (A) and Apicidin (sc-202061) treated (B) HeLa whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Histone H3 in lane B.
sc-8655
Western blot analysis of Histone H3 acetylation in untreated (A) and Oxamflatin (sc-205960) treated (B) HeLa whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Histone H3 in lane B.
sc-8655
Ac-Histone H3 (Lys 9/14): sc-8655-R. Immunofluores-cence staining of methanol-fixed HeLa cells showing nuclear localization.
sc-8655
Western blot analysis of Histone H3 acetylation in untreated (A) and Sodium Butyrate (sc-202341) treated (B) HeLa whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Histone H3 in lane B.
sc-8655
Western blot analysis of Histone H3 acetylation in untreated (A) and Valproic Acid, Sodium Salt (sc-202378) treated (B) F9 whole cell lysates. Antibody tested is Ac-Histone H3 (Lys 9/14)-R: sc-8655-R (A,B). Note acetylation of Histone H3 in lane B.
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